Method of determining toxicity with three dimensional structures

US7670797B2 · kind B2 · utility

Assignees

• The General Hospital Corporation · US
• The Charles Stark Draper Laboratory, Inc. · US

Inventors

• Joseph P. Vacanti · Winchester, US
• Robert H. Rubin · Brookline, US
• Wing Cheung · Swampscott, US
• Jeffrey T. Borenstein · Holliston, US

Key dates

• Filing date: Jul 15, 2005
• Grant date: Mar 2, 2010
• Expiry date: Sep 23, 2025

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B33Y80/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method of determining toxicity of a test agent in a tissue including A) incubating a test agent within a three-dimensional tissue engineered structure comprising a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, and wherein the first mold or polymer scaffold has microchannels comprising vessels that bifurcate, and B) obtaining information from step A) to assess toxicity.