Fluorescent dyes and complexes

US7674918B2 · kind B2 · utility

Assignee

• Cambridge Enterprise Limited · GB

Inventors

• Gerald Smith · Easton, US
• Darren William Watson · Dereham, GB
• Howard Paul Voorheis · Rathmines, IE

Key dates

• Filing date: Apr 6, 2005
• Grant date: Mar 9, 2010
• Expiry date: Feb 11, 2027

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/84
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A fluorescent dye comprising a xanthene-derived fluorophore having the formula (I) wherein R1, R2, R3, R4, R5 and R6 are independently selected from H, alkyl, alkoxy, alcohol, ether, alkenyl, alkenoxy, aryl, alkaryl, aralkyl and amido, except that R1, R4 and/or R5 is not H when bonded to Y, Y1 or Y2, respectively; X is either O− or S−; and at least one of Y, Y1, Y2, Y3, Y4 and Y5 is a group for covalently bonding the dye, optionally through. the use of a coupling agent, to a target molecule, and is otherwise H. The dye may be covalently attached to a target molecule to form a complex, and the dye and/or complex finds use in cell analysis techniques, particularly pH measurement and analysis of kinetics of migration.