Device for simultaneous multiple and high parallel synthesis

US7678335B2 · kind B2 · utility

Assignee

• Protein Technologies, Inc. · US

Inventors

• Oliver Kreuzer · Berlin, DE
• Carsten Hessenius · Berlin, DE
• Carsten Grotzinger · Berlin, DE
• Volker Johannson · Hamburg, DE
• Ralph-Th. Roeder · Chernex, CH

Key dates

• Filing date: Apr 25, 2002
• Grant date: Mar 16, 2010
• Expiry date: May 2, 2025

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2035/00158
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This invention relates to an apparatus for simultaneously carrying out multiple and highly parallel synthesis of compounds based on the solid-phase synthesis technique, in particular, peptide synthesis.Time-consuming and costly multiple rinsing processes required in solid-phase synthesis after distributing and replacing the reagents can be avoided by proposing an improved apparatus for fully automatic synthesis and subsequent separation of the synthesized compounds that involves separate synthesizer pins 11 for each synthetic unit with a reagent reservoir 20; 21 and individual dosing capability. These synthesizer pins 11 are provided in a separate holder 4 from where they are picked up by the gripper arm 2 of the automatic synthesizer 1. The openings of the reaction chambers 9 of the synthesizer plates 5 are closed (covered) by a permeable material 25 for contamination-free placement of the synthesizer pins 11 and adding reagent doses to the solid or liquid phases. In addition, a sample plate 27 that collects the samples dissolved after separation is provided underneath the valve block 6 (see FIG. 1).