Charge tags and the separation of nucleic acid molecules

US7678542B2 · kind B2 · utility

Assignee

• Third Wave Technologies, Inc. · US

Inventors

• Victor Lyamichev · Madison, US
• Zbigniev Skrzypczynski · Verona, US
• Hatim Allawi · Madison, US
• Sarah R. Wayland · Madison, US
• Tsetska Y. Takova · Madison, US
• Bruce Neri · Madison, US
• James R. Prudent · Madison, US
• Jeff G. Hall · Madison, US

Key dates

• Filing date: Jun 3, 2008
• Grant date: Mar 16, 2010
• Expiry date: Jun 3, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/683
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to novel phosphoramidites, including positive and neutrally charged compounds. The present invention also provides charge tags for attachment to materials including solid supports and nucleic acids, wherein the charge tags increase or decrease the net charge of the material. The present invention further provides methods for separating and characterizing molecules based on the charge differentials between modified and unmodified materials.