Method and apparatus for improved luminescence assays using microparticles
US7682788B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 2, 2004 |
| Grant date | Mar 23, 2010 |
| Priority date | — |
| Expiry date | Jul 2, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2458/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and apparatus for performing electrochemiluminescence assays are presented. In exemplary embodiments of the present invention an apparatus comprises an ECL-inactive electrode surface having a first assay-ligand immobilized thereon and an ECL-active microparticle having a second assay ligand immobilized thereon. In exemplary embodiments of the present invention a method comprises (a) forming a mixture comprising an electrochemiluminescent label and a microparticle having a first assay-ligand immobilized thereon, (b) applying electrochemical energy to an electrode surface in the presence of said mixture, and (c) measuring emitted electrochemiluminescence, where the microparticle is ECL-active and where the electrode surface is ECL-inactive when electrochemical energy is applied to it in the presence of said mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.