Method for producing cell culture substrate and apparatus for producing cell culture substrate
US7687251B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 24, 2005 |
| Grant date | Mar 30, 2010 |
| Priority date | — |
| Expiry date | May 19, 2026 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/703
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for producing a cell culture substrate to which cells adhere in a finely processed pattern while retaining the pattern for a long period of time to culture the cells and a production apparatus used in the production method. The method includes: forming a patterning substrate by forming: on a base material, a light shielding portion, and a cell adhesive layer having adhesion to a cell and containing a cell adhesive material which is decomposed or denatured by an action of a photocatalyst upon energy irradiation so as to cover the light shielding portion; an energy irradiating process of irradiating energy to the patterning substrate from the base material side to form a pattern; and a cell adhesion process of making the cell adhere to the cell adhesion portion in a cell culture medium containing the cell and a culture medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.