Integrated system for high throughput capture of genetic diversity
US7689366B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 11, 2003 |
| Grant date | Mar 30, 2010 |
| Priority date | — |
| Expiry date | Aug 5, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and methods for rapid and highly efficient characterization of genetic diversity in organisms are provided. The methods involve rapid sequencing and characterization of extrachromosomal DNA, particularly plasmids, to identify useful nucleotide sequences. The method involves generating a library of extrachromosomal DNA clones, sequencing a portion of the clones, comparing the sequences against a database of existing DNA sequences, using an algorithm to select novel nucleotide sequences based on the presence or absence of the sequence in a database, and identification of at least one novel nucleotide sequence. The DNA sequence can also be translated in all six frames and the resulting amino acid sequences compared against a database of protein sequences. Organisms of particular interest include, but are not limited to bacteria, fungi, algae, and the like. Compositions comprise a mini-cosmid vector comprising a stuffer fragment and at least one cos site.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.