Method for the manufacture of nucleic acid molecules

US7695906B2 · kind B2 · utility

Inventors

• Octavian Schatz · Markt Indersdorf, DE
• Timothy O'Connell · Fürstenfeldbruck, DE
• Heinz Schwer · Buchloe, DE
• Thomas Waldmann · Silver Spring, US

Key dates

• Filing date: Oct 17, 2003
• Grant date: Apr 13, 2010
• Expiry date: Nov 8, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/66
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention is related to a method for the manufacture of a nucleic acid molecule and compounds used therefore. The invention further provides a method of ligating, cleaving and immobilising oligonucleotides in order to manufacture nucleic acid molecules. The invention includes the steps wherein a first and second at least partially double-stranded oligonucleotides are ligated via their respective single-stranded overhangs. The ligation product may be immobilised to the surface via the modification that is provided on the first oligonucleotide. The immobilised ligation product is cleaved with the first type IIS restriction enzyme therein releasing an elongated oligonucleotide having an overhang. The elongated oligonucleotide may further be combined and ligated with a further at least partially double-stranded oligonucleotide to form a further ligated product that may be cleaved with a type IIS restriction enzyme releasing an elongated oligonucleotide having an overhang. The steps may be further repeated in various combinations.