Multiplexed polymerase chain reaction for genetic sequence analysis
US7695941B2 · kind B2 · utility
4Cited by
8References
24Claims
0Family size
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Key dates
| Filing date | Jun 6, 2006 |
| Grant date | Apr 13, 2010 |
| Priority date | — |
| Expiry date | Jun 6, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A PCR method involving: providing a biological sample suspected of containing one or more pathogen nucleic acids; adding a plurality of PCR primers corresponding to genes found in the pathogens; and performing a polymerase chain reaction on the sample to amplify a subset of the nucleic acids that correspond to the genes. The primers include at least one primer pair for each pathogen, and the primers contain a tail sequence that is not homologous any pathogen DNA or to any background DNA in the sample. The concentration of at least one primer in the polymerase chain reaction is no more than about 100 nM.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.