Patent · US Active

Kits for multiple non-cross reacting recombination reactions utilizing loxP sequences

US7696335B2 · kind B2 · utility

0Cited by
18References
14Claims
0Family size

Assignee

Inventors

Key dates

Filing dateOct 12, 2006
Grant dateApr 13, 2010
Priority date—
Expiry dateJul 16, 2028

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2800/30
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The invention provides methods, kits, and compositions comprising novel mutant loxP sites. Such sites are particularly useful for procedures requiring multiple site-specific recombination reactions, including deletions or insertions of multiple genes or other sequences in the same organism, staged insertions or deletions of genes of the same organism at different times, assembly of large polynucleotide constructs by serial site-specific recombination, and the like. In one aspect, compositions of the invention includes particular mutant spacer oligonucleotides of loxP recombination elements, the recombination elements themselves, and pairs of non-promiscuous mutant loxP sites.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.