Methods and compositions for highly efficient transformation of filamentous fungi
US7700349B2 · kind B2 · utility
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Key dates
| Filing date | Sep 30, 2004 |
| Grant date | Apr 20, 2010 |
| Priority date | — |
| Expiry date | Jul 2, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as Agaricus bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.