Fluorescent labeling of specific protein targets in vitro and in vivo
US7700375B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 16, 2005 |
| Grant date | Apr 20, 2010 |
| Priority date | — |
| Expiry date | Jun 16, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
New methods are provided for the post-genomic era that will permit the analysis of the dynamic expression and localization of gene products in living cells. Herein we propose the development of such a method from a bioorganic approach involving organic synthesis and protein engineering. Specifically, novel compounds bearing two maleimide groups attached directly to fluorescent cores will be prepared, whose latent fluorescence is quenched until their maleimide groups undergo a specific thiol addition reaction. Complementary a-helical proteins are designed bearing two cysteine residues appropriately positioned to react with our novel fluorogens. Genetically fusing our helical probe peptides to test proteins of interest, we can selectively label the target sequence in living cells with our small synthetic fluorogenic molecules. The scope of this technique is described in the context of studying protein localization and protein-protein interactions in living cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.