Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses
US7700744B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 30, 2005 |
| Grant date | Apr 20, 2010 |
| Priority date | — |
| Expiry date | Jun 20, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5434
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.