Monomeric streptavidin muteins
US7704708B2 · kind B2 · utility
8Cited by
5References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 11, 2007 |
| Grant date | Apr 27, 2010 |
| Priority date | — |
| Expiry date | Jul 11, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/543
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention includes a streptavidin mutein having at least one mutation chosen to cause one or more of steric hindrance, charge repulsion, or improvement of solubility by changing interfacial hydrophobic residues to less hydrophobic or hydrophilic residues. The mutein may exist in monomeric form even in the presence of biotin, and reversibly binds to biotin. The invention also includes polynucleotides encoding such muteins, expression systems and host cells for producing such muteins. The invention also includes a method of capturing biotinylated molecules using the muteins of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.