Magnetic bead agglomerator for automated ELISA process
US7713752B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 25, 2003 |
| Grant date | May 11, 2010 |
| Priority date | — |
| Expiry date | Oct 2, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54326
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Mixing of a sample containing a suspect bioagent or antigen with 1° recognition molecule coated magnetic beads in the preliminary stage of an enzyme linked immunoassay (“ELISA”) procedure is emulated by first inserting the coated magnetic beads into a non-magnetic confinement region, generating a magnetic field B along the axis of said non-magnetic confinement region to agglomerate the coated magnetic beads into a porous mass; then percolating or otherwise forcing the sample solution through said porous mass; and withdrawing the magnetic field to de-agglomerate said coated magnetic beads. In seeping or otherwise flowing through the magnetic beads the molecules of the sample link to respective magnetic beads that are coated with recognition molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.