Method of antibody purification

US7714112B2 · kind B2 · utility

Assignee

• GE Healthcare Bio-Science AB · US

Inventors

• Carina Engstrand · Uppsala, SE
• Annika Forss · Uppsala, SE
• Gunnar Glad · Uppsala, SE
• Bo-Lennart Johansson · Uppsala, SE
• Hans J. Johansson · Uppsala, SE
• Jean-Luc Maloisel · Uppsala, SE

Key dates

• Filing date: Oct 21, 2005
• Grant date: May 11, 2010
• Expiry date: Oct 25, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K16/065
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The present invention relates to a method of separating antibodies from other compound(s) in a liquid sample, wherein a mobile phase comprising said sample is contacted with a multi-modal separation matrix to adsorb undesired compounds while the antibodies remain free in the liquid, wherein the multi-modal separation matrix comprises first groups, which are capable of interacting with negatively charged sites of the target compounds, and second groups, which are capable of at least one interaction other than charge-charge interaction with said target compounds. The invention also relates to a chromatography column packed with the above-described multi-modal separation matrix and a filter having such multi-modal groups adsorbed to its surface.