Expression vector for secreting antibody fragment using E. coli signal sequence and method for mass-producing antibody fragment
US7718399B2 · kind B2 · utility
5Cited by
1References
19Claims
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Key dates
| Filing date | Oct 14, 2004 |
| Grant date | May 18, 2010 |
| Priority date | — |
| Expiry date | Sep 18, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A recombinant expression vector capable of expressing and secreting an antibody fragment fused with E. coli thermostable enterotoxin signal sequence derivative or E. coli outer membrane protein A (Omp A) signal sequence in the form of a soluble heterozygote protein is used to mass-produce the antibody fragment by culturing a microorganism transformed with the expression vector in a medium and collecting the antibody fragment secreted from the transformed microorganism into the medium.
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