Method for cellular lysis of prokaryotes or eukaryotes or simultaneous cellular lysis of prokaryotes and eukaryotes
US7723095B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 26, 2001 |
| Grant date | May 25, 2010 |
| Priority date | — |
| Expiry date | Mar 26, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M47/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for lysing prokaryotic or eukaryotic cells, or for simultaneous lysis of both, which includes at least three of the following: a mass of active, small-diameter beads corresponding to 50% or less than a mass of active, larger-diameter beads, and/or a total mass of lysing beads (consisting of a single type of bead or a mixture of smaller and larger beads) corresponding to between 50 and 100% of the total mass of the processed biological sample, and/or a lysis time of from 10 to 20 minutes, and/or seven or less non-lysing glass beads to drive the movement of the lysing beads, and/or from five to fifteen non-lysing iron beads to drive the movement of the lysing beads, depending on whether sonication, mechanical vortex centrifugation or magnetic vortex centrifugation is used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.