Method of expressing small peptides using cereal non-storage proteins as fusion carrier in endosperm and the use thereof
US7723571B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 6, 2007 |
| Grant date | May 25, 2010 |
| Priority date | — |
| Expiry date | Jun 6, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/65
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method of using cereal non-storage protein as fusion carrier to highly express small peptides in host endosperm cells. The method includes the steps of providing an endosperm-specific promoter and a DNA leading sequence encoding an endosperm-specific signal peptide; providing the gene of a non-storage protein as fusion carrier and an target gene; constructing a expression vector containing the promoter and DNA leading sequence, the gene of the fusion carrier, and a target gene; and expressing the expression vector in a host endosperm cell. Also provided in the invention are a vector constructed there from and the use thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.