Method of de novo sequencing of peptide using the MALDI mass spectrometry, method and apparatus for preparing sample for MALDI mass spectrometry
US7723917B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 8, 2006 |
| Grant date | May 25, 2010 |
| Priority date | — |
| Expiry date | Sep 27, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6851
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method of peptide sequencing by MALDI (matrix-assisted laser desorption ionization) tandem mass spectrometry, which comprises the steps of chemically modifying a sample peptide with at least one chemical modification method selected from the group consisting of guanidination and esterification in order to change the ionization status of the peptide and performing mass spectrometry using a MALDI tandem mass spectrometer and programmed MALDI tandem mass spectrometry. The peptide sequencing method of the present invention is advantageous in that detection sensitivity of the peptide improves significantly and various daughter ions are detected uniformly, thereby enabling perfect de novo sequencing with tandem mass spectrometry only, without database search.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.