Droplet-based pyrosequencing

US7727723B2 · kind B2 · utility

Assignees

• Advanced Liquid Logic, Inc. · US
• Duke University · US
• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Michael G. Pollack · Durham, US
• Vamsee K. Pamula · Durham, US
• Dwayne Allen · Durham, US
• Richard B. Fair · Durham, US
• Peter Griffin · Woodside, US

Key dates

• Filing date: Dec 15, 2006
• Grant date: Jun 1, 2010
• Expiry date: Sep 30, 2028

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/25
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to a droplet microactuator and to systems, apparatuses and methods employing the droplet microactuator for executing various protocols using droplets. The invention includes a droplet microactuator or droplet microactuator system having one or more input reservoirs loaded with reagents for conducting sequencing protocols, such as the reagents for conducting a pyrosequencing protocol. The invention also includes a droplet microactuator or droplet microactuator system, having one or more input reservoirs loaded with a sample for conducting a pyrosequencing protocol.