Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US7727727B2 · kind B2 · utility
28Cited by
105References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 26, 2009 |
| Grant date | Jun 1, 2010 |
| Priority date | — |
| Expiry date | Jan 26, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.