Controlled translocation of a polymer in an electrolytic sensing system
US7731826B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 16, 2007 |
| Grant date | Jun 8, 2010 |
| Priority date | — |
| Expiry date | Jul 28, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An electrolytic sensing system for measuring a blocking signal allows for controlled translocation of a molecule, such as DNA, through a fluid channel. A substantially constant electric field supplied by a DC source is applied across the fluid channel and induces translocation of the molecule within the system. An oscillating electric parameter (e.g. current or voltage) supplied by an AC source is also applied across the fluid channel as a means for measuring a blocking signal. The substantially constant electric field can be altered to provide more detailed control of the molecule and, optionally, run a select portion of the molecule through the channel multiple times to provide numerous signal readings. A temperature control stage cools the system, providing further control of molecule translocation. A modified or non-modified protein pore may be utilized in the fluid channel. The system allows for long DNA strands to be sequenced quickly without amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.