Method for expression of small antiviral RNA molecules within a cell

US7732207B2 · kind B2 · utility

Assignees

• CALIFORNIA INSTITUTE OF TECHNOLOGY · US
• The Regents of the University of California · US

Inventors

• Xiao-Feng Qin · Saginaw, US
• David Baltimore · Pasadena, US
• Irvin S. Y. Chen · Rancho Palos Verdes, US
• Dong Sung An · Los Angeles, US

Key dates

• Filing date: Mar 21, 2007
• Grant date: Jun 8, 2010
• Expiry date: Aug 29, 2027

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). The methods can be used to express double stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, that interfere with a viral life cycle by down regulating either the viral genome, a viral genome transcript, or a host cell that. In another aspect the invention provides methods for treating patients having suffering from infection, particularly infection with HIV. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle.