Methods for producing members of specific binding pairs
US7732377B2 · kind B2 · utility
2Cited by
49References
9Claims
0Family size
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Key dates
| Filing date | Mar 18, 2004 |
| Grant date | Jun 8, 2010 |
| Priority date | — |
| Expiry date | Aug 31, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/735
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a filamentous bacteriophage particle containing DNA encoding the sbp member, wherein the sbp member has a binding domain that consists of a dAb fragment. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected filamentous bacteriophage particles for expression of the selected sbp members.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.