Altered recombinases for genome modification
US7732585B2 · kind B2 · utility
9Cited by
2References
4Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Oct 17, 2006 |
| Grant date | Jun 8, 2010 |
| Priority date | — |
| Expiry date | Oct 17, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/30
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention describes methods of identifying altered recombinases and compositions thereof, wherein at least one amino acid is different from a parent, wild-type recombinase and the altered recombinase has improved recombination efficiency towards wild-type and/or pseudo att site sequences relative to the parent, wild-type recombinase. The present invention also includes methods of modifying the genomes of cells using the altered recombinases, including methods of site-specifically integrating a polynucleotide sequence of interest in a genome of a eucaryotic cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.