Assay device and method
US7736890B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 29, 2004 |
| Grant date | Jun 15, 2010 |
| Priority date | — |
| Expiry date | Nov 16, 2025 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/118339
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An assay method is described, which comprises the steps of immobilizing a binding partner (e.g., an antigen or antibody) for an analyte to be detected (e.g., an antibody or antigen) on a portion (140) of a surface (130) of a microfluidic chamber (120,122,124); passing a fluid sample over the surface and allowing the analyte to bind to the binding partner; allowing a metal colloid, e.g., a gold-conjugated antibody, to associate with the bound analyte; flowing a metal solution, e.g., a silver solution, over the surface such as to form an opaque metallic layer; and detecting the presence of said metallic layer, e.g., by visual inspection or by measuring light transmission through the layer, conductivity or resistance of the layer, or metal concentration in the metal solution after flowing the metal solution over the surface.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.