Optically decodable microcarries, arrays and methods

US7741031B2 · kind B2 · utility

Assignee

• The Public Health Research Institute Of the City of New York, Inc. · US

Inventors

• Fred R. Kramer · Yonkers, US
• Sanjay Tyagi · New York, US
• Salvatore A. E. Marras · Roselle Park, US
• Hiyam Elhajj Trunfio · New York, US

Key dates

• Filing date: Mar 2, 2004
• Grant date: Jun 22, 2010
• Expiry date: Sep 26, 2025

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6818
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A coding scheme for microcarriers suitable for use in distributed arrays includes labeling the carriers with quenched signaling hairpin molecules with any one of three to eight distinguishable fluorophores wherein the hairpins are of at least two types, most preferably three types, that open and fluoresce differentially as a chemical or physical condition, for example temperature, is changed. Mixtures of microcarriers having immobilized capture probes can be decoded by measuring fluorescence from said fluorophores under conditions under which only one type of hairpin is open, under which two types of hairpin are open, and so on. Mixtures of coded microcarriers with capture probes are used in assays for nucleic acids utilizing microarray methods.