Method for preparing a DNA chip and use thereof
US7741042B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 19, 2004 |
| Grant date | Jun 22, 2010 |
| Priority date | — |
| Expiry date | Nov 11, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for producing a DNA chip, which comprises the steps of: (a) cloning a probe, where a linker is coupled to one or both ends of an oligonucleotide to be integrated on a slide, into a vector; (b) transforming host cells with the vector; (c) culturing the transformed host cells, to recover the probe where the linker is coupled to one or both ends of the oligonucleotides; and (d) integrating the recovered double-helical probes on a slide. Also, the present invention relates to a DNA chip for HPV diagnosis produced by the method, and a method for diagnosing the presence or genotype of HPV using the DNA chip.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.