Sequential analysis of biological samples

US7741045B2 · kind B2 · utility

Assignee

• General Electric Company · US

Inventors

• Michael John Gerdes · Albany, US
• Anup Sood · Flemington, US
• Michael Christopher Montalto · Albany, US
• Ali Can · Andover, US
• Fiona Ginty · Niskayuna, US
• Maureen Ann Bresnahan · Niskayuna, US
• Robert John Filkins · Niskayuna, US
• Zhengyu Pang · Niskayuna, US

Key dates

• Filing date: Sep 28, 2007
• Grant date: Jun 22, 2010
• Expiry date: Apr 22, 2028

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/542
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.