Patent · US Active

High throughput prion assays

US7741054B2 · kind B2 · utility

0Cited by

0References

4Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Walter Eberle · Biberach an der Riß, DE
Werner Stock · Gräfelfing, DE
Baerbel Winter · Tutzing, DE

Key dates

Filing date	Nov 14, 2005
Grant date	Jun 22, 2010
Priority date	—
Expiry date	Jun 18, 2026

Classification

Technology area (CPC G)Physics
CPC primaryG01N2800/2828
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The invention is directed to a method for performing a high throughput test to determine the presence of PrPsc in a tissue sample taken post mortem from brain, the test comprising the workflow of (A) sample preparation, (B) sample treatment, (C) sample analysis, (D) controls, and (E) classifying the results of the analysis as positive or negative. The invention is further directed to preparing a treated biological sample suspected of containing PrPsc such that it is suitable for specific detection of PrPsc, if present, the method comprising the steps of (a) homogenizing the sample; followed by (b) digesting the sample proteolytically by adding to the homogenate Proteinase K and incubating the sample; followed by (c) adding a peptidic protease inhibitor to the incubated mixture of step (b), thereby obtaining a treated biological sample suitable for specific detection of PrPsc, if present. The invention further provides a conditioned microwell plate. In addition, the invention provides a soluble fusion protein comprising one or more epitopes of prion protein as well as a carrier polypeptide.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.