Transformed plant cell expressing five mammalian proteins involved in sialylation and a protein involved in galactosylation

US7741539B2 · kind B2 · utility

Assignee

• GREENOVATION BIOTECH GMBH · DE

Inventors

• Gilbert Gorr · Glückstadt, DE
• Heike Launhardt · Glückstadt, DE
• Marta Rodriguez Franco · Glückstadt, DE
• Christian Stemmer · Glückstadt, DE

Key dates

• Filing date: Jul 12, 2006
• Grant date: Jun 22, 2010
• Expiry date: Jul 12, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8245
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for producing heterologous glycosylated proteins in non-animal eukaryotic cells such as in transformed bryophyte, yeast, ciliate or algae cells. In particular, the method relates to a method for producing glycosylated proteins comprising animal glycosylation patterns—comprising sialic acid residues—, such as pharmaceutical proteins for use in mammals, e.g. humans, in bryophyte cells such as those of Physcomitrella patens, the genetic material required therefore, such as DNA and RNA, vectors, host cells, methods of introducing genetic material there into, and uses thereof. Furthermore, the present invention relates to novel polypeptides and proteins obtained by the method according to the invention. Moreover, the present invention provides a method of producing sialic acid or CMP-sialic acid in a transformed non-mammalian eukaryotic cell, tissue or organism.