Isolation and cloning of DNA from uncultivated organisms

US7749366B2 · kind B2 · utility

Assignee

• B.R.A.I.N. Biotechnology Research and Information Network AG · DE

Inventors

• Achim Quaiser · Spaichingen, DE
• Torsten Ochsenreiter · Woods Hole, US
• Alexander H. Treusch · Messel, DE
• Arnulf Kletzin · Darmstadt, DE
• Christa Schleper · Klosterneuburg, AT
• Patrick Lorenz · Lorsch, DE
• Jürgen Eck · Bensheim, DE

Key dates

• Filing date: Aug 20, 2003
• Grant date: Jul 6, 2010
• Expiry date: Aug 17, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1003
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.