Detection of chemical ligation using fluorescence quenching leaving groups
US7749699B2 · kind B2 · utility
4Cited by
10References
47Claims
0Family size
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Inventor
Key dates
| Filing date | Jul 17, 2003 |
| Grant date | Jul 6, 2010 |
| Priority date | — |
| Expiry date | Jan 25, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6823
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Novel compounds having a fluorescence quencher as a leaving group are disclosed. Nucleic acids and other molecules containing a fluorophore and a fluorescence quencher are disclosed as an embodiment of this invention. The use of the oligonucleotides in enzyme-free oligonucleotide ligation reactions results in an increase in fluorescence when the oligonucleotide also contains a nearby fluorophore. The ligation reactions can be performed in solution, on surfaces, or in cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.