High throughput screening assay for the TRPM5 ion channel

US7749730B2 · kind B2 · utility

Assignee

• Redpoint Bio Corporation · US

Inventors

• Robert W. Bryant · Princeton, US
• S. Paul Lee · Phoenix, US
• R. Kyle Palmer · Cranbury, US
• Qifeng Yang · Montgomery, US
• M. N. Tulu Buber · Newtown, US

Key dates

• Filing date: Nov 3, 2006
• Grant date: Jul 6, 2010
• Expiry date: Jul 12, 2027

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

There exists a need in the art for high throughput screening assays that can identify compounds that specifically modulate the activity of fast-acting ion channels, such as TRPM5. Current methods suffer from a lack of sensitivity, low throughput, and are labor intensive. The claimed methods provide fluorescent assays with an optical readout that gives rapid readout of the results, has a high signal to noise background ratio, are easy to use, can be modified for automation and miniaturization, and provide verification that a compound specifically modulates TRPM5.