Optimized T-DNA transfer and vectors therefor

US7749751B2 · kind B2 · utility

Assignee

• CropDesign N.V. · BE

Inventors

• Anna Depicker · Schelderode, BE
• Vladimir Mironov · Evergem, BE
• Franky Terras · Waregem, BE
• Willem Broekaert · Dilbeek, BE
• Sylvie De Buck · Knokke, BE
• Chris De Wilde · Aalst, BE

Key dates

• Filing date: Dec 13, 2000
• Grant date: Jul 6, 2010
• Expiry date: Mar 3, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2810/65
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.