Process for the purification of antibodies
US7750129B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 25, 2005 |
| Grant date | Jul 6, 2010 |
| Priority date | — |
| Expiry date | Jun 26, 2025 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J20/3285
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.