Identification of high affinity molecules by limited dilution screening
US7754433B2 · kind B2 · utility
3Cited by
13References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 2, 2002 |
| Grant date | Jul 13, 2010 |
| Priority date | — |
| Expiry date | Oct 26, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6857
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods are disclosed for determining the relative binding affinities of molecules for their binding partner. One type of molecule is an antibody, which has an antigen binding partner. Antibodies are tested for binding against limited dilutions of antigen. Antibodies that bind to the most dilute antigen concentrations are determined to have a higher relative binding affinity for the antigen than antibodies that only bind to more concentrated antigen preparations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.