Large-scale production of human serum butyrylcholinesterase as a bioscavenger

US7754461B2 · kind B2 · utility

Assignee

• The United States of America as represented by the Secretary of the Army · US

Inventors

• Bhupendra P. Doctor · Potomac, US
• Ashima Saxena · Fairfax, US
• Wei Sun · Timonium, US
• Chunyuan Luo · North Potomac, US
• Prasanthi Tipparaju · Rockville, US
• Irwin Koplovitz · Bel Air, US
• David Lenz · Bel Air, US
• Michelle Ross · Edgewood, US

Key dates

• Filing date: Apr 10, 2007
• Grant date: Jul 13, 2010
• Expiry date: Apr 10, 2027

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Disclosed herein are methods for the large-scale preparation of human butyrylcholinesterase (HuBChE) preparations from Cohn Fraction IV-4. As disclosed, the methods provide HuBChE preparations that are about 99% or more pure with recovery yields of about 60%. Also disclosed are the pharmacokinetics, safety and toxicity, stability and efficacy of the HuBChE preparations.