High density growth of T7 expression strains with auto-induction option
US7759109B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 4, 2008 |
| Grant date | Jul 20, 2010 |
| Priority date | — |
| Expiry date | Apr 4, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A bacterial growth medium for promoting auto-induction of transcription of cloned DNA in cultures of bacterial cells grown batchwise is disclosed. The transcription is under the control of a lac repressor. Also disclosed is a bacterial growth medium for improving the production of a selenomethionine-containing protein or polypeptide in a bacterial cell, the protein or polypeptide being produced by recombinant DNA techniques from a lac or T7lac promoter, the bacterial cell encoding a vitamin B12-dependent homocysteine methylase. Finally, disclosed is a bacterial growth medium for suppressing auto-induction of expression in cultures of bacterial cells grown batchwise, said transcription being under the control of lac repressor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.