Systems and methods for efficient collection of single cells and colonies of cells and fast generation of stable transfectants

US7759119B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Nancy Allbritton · Irvine, US
• Christopher Sims · Irvine, US
• Yuli Wang · Lynnwood, US
• Mark Bachman · Irvine, US
• Guann-Pyng Li · Irvine, US
• Eric J. Stanbridge · Newport Beach, US

Key dates

• Filing date: Apr 9, 2007
• Grant date: Jul 20, 2010
• Expiry date: Apr 15, 2027

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2001/2886
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses. Use of the plate advantageously enables the selection and sorting of cells based on dynamic phenomena and the rapid establishment of stable tranfectants.