Expression system for enhancing solubility and immunogeneicity of recombinant proteins

US7759461B2 · kind B2 · utility

Assignees

• Stresspro Biomedicine Incorporation · TW
• ANIMAL TECHNOLOGY INSTITUTE TAIWAN · TW

Inventors

• Ming-Yu Chen · Hangzhou City, CN
• Chin-Kai Chuang · Gongguan, TW
• Yu-Hsyu Su · Taipei, TW
• Chiu-Ting Fan · Hengshan, TW
• Jung-Chuan Yu · Hsinchu, TW
• Wen-Chuan Lee · Hsinchu, TW

Key dates

• Filing date: Mar 18, 2009
• Grant date: Jul 20, 2010
• Expiry date: Jun 23, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2740/16322
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Expression system for enhancing solubility and immunogenicity of recombinant proteins. The expression system includes a protein expression vector that contains a chimeric gene encoding a chimeric protein comprising: (a) a first polypeptidyl fragment at the N-terminal end of the chimeric protein, containing a protein transduction domain (PTD), or a fragment thereof, having HIV Tat PTD activity; (b) a second polypeptidyl fragment at the C-terminal end of the first polypeptidyl fragment, containing a J-domain, or a fragment thereof, having heat shock protein 70 (Hsp70)-interacting activity; and (c) a third polypeptidyl fragment at the C-terminal end of the second polypeptidyl fragment, containing a target protein or polypeptide.