Labeling reagent

US7759470B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Dieter Heindl · Munich, DE
• Frank Bergmann · Iffeldorf, DE
• Hans-Peter Josel · Weilheim, DE
• Dirk Kessler · Peiting, DE
• Gunter Lampert · Wörth an der Donau, DE
• Christian Weilke · Penzberg, DE

Key dates

• Filing date: Feb 16, 2007
• Grant date: Jul 20, 2010
• Expiry date: Feb 16, 2027

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/55
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.