Detection of recombinase polymerase amplification products
US7763427B2 · kind B2 · utility
81Cited by
17References
17Claims
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Assignee
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Key dates
| Filing date | May 7, 2008 |
| Grant date | Jul 27, 2010 |
| Priority date | — |
| Expiry date | May 7, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This disclosure describe three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.