Two-photon absorbing dipyrrometheneboron difluoride dyes and their applications
US7763439B2 · kind B2 · utility
3Cited by
6References
3Claims
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Key dates
| Filing date | Jul 1, 2002 |
| Grant date | Jul 27, 2010 |
| Priority date | — |
| Expiry date | Aug 6, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A separation free bioanalytical assay method for measuring an analyte from a biological fluid or suspension containing microparticles as a bioaffinity binding solid phase, a biospecific secondary reagent labelled with a two-photon fluorescent dipyrrometheneboron difluoride dye of specified structure, focusing the laser into the reaction suspension, and measuring two-photon excited fluorescence from single microparticles when they randomly float or are guided by the radiation pressure of the excitation laser through the focal volume of the laser beam.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.