Sushi peptide multimer

US7763704B2 · kind B2 · utility

Assignee

• NATIONAL UNIVERSITY OF SINGAPORE · SG

Inventors

• Jeak Ling Ding · Singapore, SG
• Bow Ho · Singapore, SG

Key dates

• Filing date: Jul 2, 2004
• Grant date: Jul 27, 2010
• Expiry date: Feb 27, 2026

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S930/28
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Endotoxin, also known as lipopolysaccharides (LPS), is the major mediator of septic shock due to Gram-negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain of Factor C, which is the endotoxin-sensitive serine protease of the limulus coagulation cascade, binds and neutralizes LPS activity. Fluorescent tagged-S3 is shown to detect LPS-containing bacteria. For large-scale production of S3 and to mimic other pathogen-recognizing molecules, tandem multimers of the S3 gene were constructed and expressed in E. coli. Tetramer of S3 for example is shown to display an enhanced inhibitory effect on LPS-induced activities. An affinity matrix based on tetramer of S3 is also shown to be particularly efficient at removing LPS.