Degenerate oligonucleotide gene shuffling
US7771974B2 · kind B2 · utility
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3References
21Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 29, 2001 |
| Grant date | Aug 10, 2010 |
| Priority date | — |
| Expiry date | Sep 11, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for gene shuffling to form a mutant or chimeric gene, the method comprising: (a) assigning one or more segments of one or more genes based on regions of encoded amino acid sequence; (b) amplifying the one or more assigned segments of the gene using primers specific for each segment; and (c) causing recombination of the one or more amplified segments to form a mutant or chimeric gene. An oligonucleotide primer suitable for use in gene shuffling, the primer having a non-degenerate core based on a segment or template of a gene to be amplified, and the core being flanked by both 5′ and 3′ degenerate ends.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.