Extraction and quantification of vitamins A & D in fluid samples
US7776544B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 10, 2003 |
| Grant date | Aug 17, 2010 |
| Priority date | — |
| Expiry date | Apr 21, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/82
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention discloses monoclonal antibodies for vitamins A (retinol palmitate) and D3 (cholecalciferol); a method for using monoclonal antibodies, and the monoclonal antibodies disclosed herein, in particular, to quantitate these vitamins in fluids such as dairy products, and blood, and also raw or processed agri-food and beverage products. The method involves contacting the sample with a mixture of polar and non-polar organic solvents in combination with inorganic salts to remove fat molecules into an organic fraction, and assaying the organic fraction by immunoassay involving the monoclonal antibodies. The presence of a mixture of non-polar and polar organic solvents increases the separation of vitamins from fat molecules and enables the test samples to be quantified by immunoassay without any further treatment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.