Biosensor using living cells on silicon-based microarrays
US7776794B2 · kind B2 · utility
1Cited by
1References
15Claims
0Family size
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Key dates
| Filing date | Sep 20, 2004 |
| Grant date | Aug 17, 2010 |
| Priority date | — |
| Expiry date | Sep 20, 2024 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00743
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a biosensor comprising living cells that express a chemosensor, or receptor, on their surface. When grown on a microarray comprising electrodes, the cells can be induced, by binding of a ligand to the receptor, to secrete a molecule. This secretion event is detected with millisecond temporal resolution via electrochemical oxidation of the secreted molecule on the electrode which is voltage-clamped slightly above its redox potential. The current so generated is indicative of the amount of the ligand bound to the receptor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.