Process for purifying interferon beta
US7780960B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 4, 2004 |
| Grant date | Aug 24, 2010 |
| Priority date | — |
| Expiry date | Sep 3, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K5/06139
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a process for purifying human interferon beta from a recombinant human interferon beta-containing culture comprising performing affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), wherein the affinity chromatography includes: adsorbing the interferon beta-containing culture to an equilibrated affinity chromatography column, followed by washing with an equilibration buffer solution; washing the column with a washing buffer solution A of pH 6.5-7.5 containing 30-60 wt % of propylene glycol and a washing buffer solution B of pH 6.5-7.5 containing 10-30 wt % of propylene glycol and 1-2M NaCl; and eluting a human interferon beta-containing fraction with a buffer solution of pH 6.5-7.5 containing 40-60 wt % of propylene glycol and 1-2M NaCl.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.